Electronic Journal of Biotechnology ISSN: 0717-3458
  © 2007 by Universidad Católica de Valparaíso -- Chile
Vol. 10 No. 2, Issue of April 15, 2007
 

Table 1. Summary of purification of β-glucosidase from Melanocarpus sp. MTCC 3922.


Purification
step

Total activity
(units)

Total protein
(mg)

Specific activity
(Ámol min-1
mg protein -1)

Fold
purification

Yield
(% recovery)

Culture filtrate

4895.0

1975.0

2.47

-

100

Ultra- filtration

4104.0

1445.2

2.8

1.13

83.84

DEAE-Sepharose

1476.0

197.3

7.48

3.03

30.15

PBE-94

778.0

77.47

10.04

4.06

15.89


Supported by UNESCO / MIRCEN network